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ultrascript 2 0 cdna synthesis kit  (PCR Biosystems Ltd)


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    PCR Biosystems Ltd ultrascript 2 0 cdna synthesis kit
    Ultrascript 2 0 Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase+cdna+synthesis+kit/UltraScript+2%2E0+Reverse+Transcriptase/pmc13694090-75-12-17
    Average 94 stars, based on 90 article reviews
    ultrascript 2 0 cdna synthesis kit - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Synthesized:

    Article Title: Gene Expression Analysis of Galectin-9, MALAT1, and ZAP-70 in Chronic Lymphocytic Leukemia and Their Diagnostic Potential: Association with Clinical Laboratory Features
    Article Snippet: The concentration and purity of RNA were measured by OneDrop TOUCH NanoDrop Spectrophotometer (Biometrics, USA). .. 2.5.2 Complementary DNA (cDNA) cDNA was synthesized with cDNA Synthesis UltraScript Reverse Transcriptase kit from (PCRBIOSYSTEM) by using SimpliAmp Thermal Cycler 96-WELL (Applied Biosystems), in a 20 μL reaction volume containing 4 μL of 5× cDNA synthesis mix, 1 μL of UltraScript for cDNA synthesis, 10 μL of PCR-grade water, and 5 μL of extracted RNA. .. 2.5.3 Quantitative real-time PCR (qRT-PCR) The transcript levels of three different target genes (Galectin-9, Zap-70, and MALAT1) were assessed by operating the reactions in the BioRad (CFX 96) Touch Real-Time PCR Detection System (Bio-Rad, USA).

    Article Title: NQO1 acts as a context-dependent regulator of oxidative stress and biliary fibrosis in primary sclerosing cholangitis
    Article Snippet: The concentration and integrity of the extracted RNA were assessed via NanoDrop spectrophotometer (NanoDrop Technologies, DE, USA). .. Subsequently, 1 μg of total RNA was synthesized into cDNA using the UltraScript 2.0 cDNA Synthesis Kit (PCR BioSystems, London, UK). .. Quantitative PCR was conducted using the AccuPower 2× Greenstar qPCR Master Mix (Bioneer, Daejeon, Korea) on a StepOnePlus Real-Time PCR system (Applied Biosystems, CA, USA).

    cDNA Synthesis:

    Article Title: Gene Expression Analysis of Galectin-9, MALAT1, and ZAP-70 in Chronic Lymphocytic Leukemia and Their Diagnostic Potential: Association with Clinical Laboratory Features
    Article Snippet: The concentration and purity of RNA were measured by OneDrop TOUCH NanoDrop Spectrophotometer (Biometrics, USA). .. 2.5.2 Complementary DNA (cDNA) cDNA was synthesized with cDNA Synthesis UltraScript Reverse Transcriptase kit from (PCRBIOSYSTEM) by using SimpliAmp Thermal Cycler 96-WELL (Applied Biosystems), in a 20 μL reaction volume containing 4 μL of 5× cDNA synthesis mix, 1 μL of UltraScript for cDNA synthesis, 10 μL of PCR-grade water, and 5 μL of extracted RNA. .. 2.5.3 Quantitative real-time PCR (qRT-PCR) The transcript levels of three different target genes (Galectin-9, Zap-70, and MALAT1) were assessed by operating the reactions in the BioRad (CFX 96) Touch Real-Time PCR Detection System (Bio-Rad, USA).

    Article Title: Coat Color‐Associated Transcriptomic Differences of Black and White Hortobágyi Racka Sheep Using the RNA‐Seq Technique
    Article Snippet: Primer Express v3.0.1 software (Applied Biosystems, Foster City, CA, USA) was used to design the primers and was validated using Primer Blast from NCBI (Ye et al. ) (Table ). .. The qPCRBIO cDNA Synthesis Kit (PCR Biosystems, London, UK) was used. ..

    Article Title: NQO1 acts as a context-dependent regulator of oxidative stress and biliary fibrosis in primary sclerosing cholangitis
    Article Snippet: The concentration and integrity of the extracted RNA were assessed via NanoDrop spectrophotometer (NanoDrop Technologies, DE, USA). .. Subsequently, 1 μg of total RNA was synthesized into cDNA using the UltraScript 2.0 cDNA Synthesis Kit (PCR BioSystems, London, UK). .. Quantitative PCR was conducted using the AccuPower 2× Greenstar qPCR Master Mix (Bioneer, Daejeon, Korea) on a StepOnePlus Real-Time PCR system (Applied Biosystems, CA, USA).

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    Article Title: Sex-specific division of labor in termites is linked to differential brain architecture and foraging gene expression
    Article Snippet: Illustra GFX PCR DNA and Gel Band Purification Kit , Cytiva , Cat#28903470. .. Ultrascript cDNA Synthesis Kit , PCR Biosystems , PB30.11–10. .. qPCRBIO SyGreen Mix Separate-Rox , PCR Biosystems , PB20.14–05.

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli .
    Article Snippet: RNA samples were treated with DNase I (Qiagen, Valencia, CA) to eliminate genomic DNA. .. 0.4 μg of total purified RNA was reverse transcribed using a qPCRBIO cDNA synthesis kit (PCR Biosystems, London, UK). .. Quantitative Real-Time PCR (qRT-PCR) was run for 40 cycles on an Eppendorf Mastercycler ep Realplex with a mix of 1 μL (10 ng) cDNA, 1 μL each of forward and reverse primers (10 μM stock), and 10 μL of SyGreen Mix Lo-ROX (PCR Biosystems, London, UK).

    Reverse Transcription:

    Article Title: Gene Expression Analysis of Galectin-9, MALAT1, and ZAP-70 in Chronic Lymphocytic Leukemia and Their Diagnostic Potential: Association with Clinical Laboratory Features
    Article Snippet: The concentration and purity of RNA were measured by OneDrop TOUCH NanoDrop Spectrophotometer (Biometrics, USA). .. 2.5.2 Complementary DNA (cDNA) cDNA was synthesized with cDNA Synthesis UltraScript Reverse Transcriptase kit from (PCRBIOSYSTEM) by using SimpliAmp Thermal Cycler 96-WELL (Applied Biosystems), in a 20 μL reaction volume containing 4 μL of 5× cDNA synthesis mix, 1 μL of UltraScript for cDNA synthesis, 10 μL of PCR-grade water, and 5 μL of extracted RNA. .. 2.5.3 Quantitative real-time PCR (qRT-PCR) The transcript levels of three different target genes (Galectin-9, Zap-70, and MALAT1) were assessed by operating the reactions in the BioRad (CFX 96) Touch Real-Time PCR Detection System (Bio-Rad, USA).

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli .
    Article Snippet: RNA samples were treated with DNase I (Qiagen, Valencia, CA) to eliminate genomic DNA. .. 0.4 μg of total purified RNA was reverse transcribed using a qPCRBIO cDNA synthesis kit (PCR Biosystems, London, UK). .. Quantitative Real-Time PCR (qRT-PCR) was run for 40 cycles on an Eppendorf Mastercycler ep Realplex with a mix of 1 μL (10 ng) cDNA, 1 μL each of forward and reverse primers (10 μM stock), and 10 μL of SyGreen Mix Lo-ROX (PCR Biosystems, London, UK).

    Polymerase Chain Reaction:

    Article Title: Gene Expression Analysis of Galectin-9, MALAT1, and ZAP-70 in Chronic Lymphocytic Leukemia and Their Diagnostic Potential: Association with Clinical Laboratory Features
    Article Snippet: The concentration and purity of RNA were measured by OneDrop TOUCH NanoDrop Spectrophotometer (Biometrics, USA). .. 2.5.2 Complementary DNA (cDNA) cDNA was synthesized with cDNA Synthesis UltraScript Reverse Transcriptase kit from (PCRBIOSYSTEM) by using SimpliAmp Thermal Cycler 96-WELL (Applied Biosystems), in a 20 μL reaction volume containing 4 μL of 5× cDNA synthesis mix, 1 μL of UltraScript for cDNA synthesis, 10 μL of PCR-grade water, and 5 μL of extracted RNA. .. 2.5.3 Quantitative real-time PCR (qRT-PCR) The transcript levels of three different target genes (Galectin-9, Zap-70, and MALAT1) were assessed by operating the reactions in the BioRad (CFX 96) Touch Real-Time PCR Detection System (Bio-Rad, USA).

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    other:

    Article Title: H 2 O 2 repurposes plant O 2 sensing to regulate post-hypoxia responses.
    Article Snippet: The protein signal was visualized by chemiluminescence (ECL Plus, Pierce).

    Real-time Polymerase Chain Reaction:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    SYBR Green Assay:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    Purification:

    Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli .
    Article Snippet: RNA samples were treated with DNase I (Qiagen, Valencia, CA) to eliminate genomic DNA. .. 0.4 μg of total purified RNA was reverse transcribed using a qPCRBIO cDNA synthesis kit (PCR Biosystems, London, UK). .. Quantitative Real-Time PCR (qRT-PCR) was run for 40 cycles on an Eppendorf Mastercycler ep Realplex with a mix of 1 μL (10 ng) cDNA, 1 μL each of forward and reverse primers (10 μM stock), and 10 μL of SyGreen Mix Lo-ROX (PCR Biosystems, London, UK).



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